產(chǎn)品編號(hào) | bsm-43099M |
英文名稱 | Mouse Anti-PRL antibody |
中文名稱 | 泌乳素單克隆抗體 |
別 名 | Luteotropic Hormone; Mammotropin; OTTHUMP00000017727; PRL; Prolactin; Prolactin precursor; PRL_HUMAN |
研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 生長因子和激素 激酶和磷酸酶 內(nèi)分泌病 |
抗體來源 | Mouse |
克隆類型 | Monoclonal |
交叉反應(yīng) | Human,Rat |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 24kDa |
細(xì)胞定位 | 分泌型蛋白 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | Recombinant human PRL protein: 29-227/227 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | PBS. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]. SWISS: P01236 Gene ID: 5617 Database links: Entrez Gene: 5617 Human Entrez Gene: 19109 Mouse Omim: 176760 Human SwissProt: P01236 Human SwissProt: P06879 Mouse |
產(chǎn)品圖片 |
Sample:
Lane 1: Rat Pituitary tissue lysates
Primary: Anti-PRL (bsm-43099M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 23 kDa
Paraformaldehyde-fixed, paraffin embedded (human pituitary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRL) Monoclonal Antibody, Unconjugated (bsm-43099M) at 1:300 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human pituitary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRL ) Monoclonal Antibody, Unconjugated (bsm-43099M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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