| 產(chǎn)品編號 | bs-41365R |
| 英文名稱 | Rabbit Anti-Beclin 1 antibody |
| 中文名稱 | 自噬效應(yīng)蛋白Beclin 1抗體 |
| 別 名 | Beclin-1; ATG6; beclin1; VPS30; Beclin 1 like protein 1; 4921513J16Rik; 5430417M23Rik; ATG6; BECLIN 1; BECN1; MGC6843; BECN1_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 免疫學 神經(jīng)生物學 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Beclin 1 protein : 141-450/450 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl 2 binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 52kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons. SWISS: Q14457 Gene ID: 8678 Database links: Entrez Gene: 8678 Human Entrez Gene: 56208 Mouse Omim: 604378 Human SwissProt: Q14457 Human SwissProt: O88597 Mouse Unigene: 716464 Human Unigene: 178947 Mouse Unigene: 2776 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Recombinant human Beclin 1 protein, N-His(bs-41365P)
Primary: Anti-Beclin 1 (bs-41365R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 38 kDa
Sample:
Lane 1: Human Raji cell lysates
Lane 2: Human HeLa cell lysates
Lane 3: Human A431 cell lysates
Lane 4: Human HepG2 cell lysates
Lane 5: Human MCF-7 cell lysates
Primary: Anti-Beclin 1 (bs-41365R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 55 kDa
Measured by its binding ability in a indirect ELISA. Immobilized Human Beclin 1 protein, His Tag (Cat. bs-41365P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human Beclin 1 Antibody, the EC50 is 2253 ng/mL.
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
