| 產(chǎn)品編號 | bs-41346R |
| 英文名稱 | Rabbit Anti-ACAN antibody |
| 中文名稱 | 軟骨蛋白聚糖抗體 |
| 別 名 | Aggrecan; Aggrecan core protein; Cartilage-specific proteoglycan core protein; CSPCP; Chondroitin sulfate proteoglycan core protein 1; Chondroitin sulfate proteoglycan 1; Aggrecan core protein 2; AGC1; CSPG1; MSK16; PGCA_HUMAN. |
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Specific References (2) | bs-41346R has been referenced in 2 publications.
[IF=5.714] Yuhan Qu. et al. Chicoric acid attenuates tumor necrosis factor-α-induced inflammation and apoptosis via the Nrf2/HO-1, PI3K/AKT and NF-κB signaling pathways in C28/I2 cells and ameliorates the progression of osteoarthritis in a rat model. INT IMMUNOPHARMACOL. 2022 Oct;111:109129 WB ; Human.
[IF=4.657] Shen, Yue. et al. Hederagenin Suppresses Inflammation and Cartilage Degradation to Ameliorate the Progression of Osteoarthritis: An In vivo and In vitro Study. INFLAMMATION. 2022 Nov;:1-24 WB ; Human.
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| 研究領(lǐng)域 | 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 干細(xì)胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 222kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human ACAN protein: 2321-2530/2530 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS. Function: Aggrecan has been detected in neural precursor cells (neurospheres; Kabos et al, 2004) During differentiation, neurospheres downregulate Chondroitin sulfate proteoglycans (CSPGs). Proliferating neural precursors synthesize lecticans, including aggrecan, which are downregulated with differentiation; suggesting a link between CSPGs and CNS precursor biology. Subunit: Interacts with FBLN1. Interacts with COMP. Subcellular Location: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Restricted to cartilages. Post-translational modifications: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase. DISEASE: Spondyloepiphyseal dysplasia type Kimberley (SEDK) [MIM:608361]: Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy. Note=The disease is caused by mutations affecting the gene represented in this entry. Spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN) [MIM:612813]: A bone disease characterized by severe short stature, macrocephaly, severe midface hypoplasia, short neck, barrel chest and brachydactyly. The radiological findings comprise long bones with generalized irregular epiphyses with widened metaphyses, especially at the knees, platyspondyly, and multiple cervical-vertebral clefts. Note=The disease is caused by mutations affecting the gene represented in this entry. Osteochondritis dissecans short stature and early-onset osteoarthritis (OD) [MIM:165800]: A type of osteochondritis defined as a separation of cartilage and subchondral bone from the surrounding tissue, primarily affecting the knee, ankle and elbow joints. It is clinically characterized by multiple osteochondritic lesions in knees and/or hips and/or elbows, disproportionate short stature and early-onset osteoarthritis. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the aggrecan/versican proteoglycan family. Contains 1 C-type lectin domain. Contains 1 EGF-like domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. Contains 4 Link domains. Contains 1 Sushi (CCP/SCR) domain. SWISS: P16112 Gene ID: 176 Database links: Entrez Gene: 176 Human Entrez Gene: 11595 Mouse Omim: 155760 Human SwissProt: P16112 Human SwissProt: Q61282 Mouse Unigene: 2159 Human Unigene: 358571 Mouse Unigene: 54503 Rat Aggrecan是軟骨細(xì)胞外基質(zhì)的主要結(jié)構(gòu)成分之一。它與膠原蛋白網(wǎng)絡(luò)結(jié)合，維持軟骨彈性、緩沖壓力，承擔(dān)負(fù)荷,并有自我潤滑的性能。蛋白多糖的進(jìn)行性喪失是骨性關(guān)節(jié)病發(fā)病主要原因之一。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Recombinant human ACAN protein
Primary: Anti-ACAN (bs-41346R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 222 kDa
Observed band size: 27 kDa
Measured by its binding ability in a indirect ELISA. Immobilized Human ACAN protein, His Tag (Cat. bs-41346P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human ACAN Antibody, the EC50 is 3071 ng/mL.
Measured by its binding ability in a indirect ELISA. Immobilized Human ACAN protein, His Tag (Cat. bs-41346P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human ACAN Antibody, the EC50 is 402 ng/mL.
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