| 產品編號 | bs-25093R |
| 英文名稱 | Rabbit Anti-PAX1 antibody |
| 中文名稱 | 配對盒基因1抗體 |
| 別 名 | HUP48; OTTHUMP00000030407; Paired box 1; Paired box gene 1; Paired box protein Pax 1; Paired domain gene HuP48; PAX 1; PAX1_HUMAN. |
| 研究領域 | 腫瘤 發(fā)育生物學 信號轉導 干細胞 轉錄調節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Dog) |
| 產品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PAX1: 181-280/534 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. This gene plays a role in pattern formation during embryogenesis and may be essential for development of the vertebral column. This gene is silenced by methylation in ovarian and cervical cancers and may be a tumor suppressor gene. Mutations in this gene are also associated with vertebral malformations. [provided by RefSeq, Mar 2012] Function: This protein is a transcriptional activator. It may play a role in the formation of segmented structures of the embryo. May play an important role in the normal development of the vertebral column. Subcellular Location: Nucleus. Similarity: Contains 1 paired domain. SWISS: P15863 Gene ID: 5075 Database links: Entrez Gene: 5075 Human Entrez Gene: 18503 Mouse Omim: 167411 Human SwissProt: P15863 Human SwissProt: P09084 Mouse Unigene: 349082 Human Unigene: 315502 Mouse 同源結構域蛋白（Homeodomain Proteins） PAX蛋白在器官發(fā)生中起關鍵作用，包括促進細胞增殖、細胞世系的特異化、遷移和存活。一些PAX基因在多種癌癥類型中表達，因此可以作為有用的腫瘤標志物。 |
| 產品圖片 |
Sample:
Lane 1: Mouse Thymus Lysates
Lane 2: Mouse Embryo Lysates
Lane 3: Human U-2 OS cell Lysates
Lane 4: Human HL-60 cell Lysates
Lane 5: Human Raji cell Lysates
Primary: Anti-PAX1 (bs-25093R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55kDa
Observed band size: 60kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
