| 產(chǎn)品編號 | bsm-51691M |
| 英文名稱 | Mouse Anti-HACE1 antibody |
| 中文名稱 | E3泛素蛋白連接酶HACE1單克隆抗體 |
| 別 名 | E3 ubiquitin-protein ligase HACE1; HACE 1; HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; KIAA1320; HACE1_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 染色質(zhì)和核信號 細(xì)胞周期蛋白 表觀遺傳學(xué) |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | B4F6 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human HACE1. |
| 亞 型 | IgG1,k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a HECT domain and ankyrin repeat-containing ubiquitin ligase. The encoded protein is involved in specific tagging of target proteins, leading to their subcellular localization or proteasomal degradation. The protein is a potential tumor suppressor and is involved in the pathophysiology of several tumors, including Wilm's tumor. [provided by RefSeq, Mar 2016] Function: E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB. Subunit: Interacts with RARB (By similarity). Interacts with RAB1 (RAB1A, RAB1B or RAB1C), RAB4 (RAB4A or RAB4B) and RAB11 (RAB11A or RAB11B); in a GTP-dependent manner. Interacts with RAC1; in a GTP-dependent manner. Interacts with the 26S proteasomal complex through the 20S core proteasomal subunit. Subcellular Location: Cytoplasm. Endoplasmic reticulum. A significant portion localizes to the endoplasmic reticulum. Tissue Specificity: Expressed in multiple tissues including heart, brain and kidney. DISEASE: Note=Defects in HACE1 are a cause of Wilms tumor (WT). WT is a pediatric malignancy of kidney and one of the most common solid cancers in childhood. HACE1 is epigenetically down-regulated in sporadic Wilms tumor. Moreover, a t(5;6)(q21;q21) translocation that truncates HACE1 has been found in a child with bilateral, young-onset Wilms tumor. Similarity: Contains 6 ANK repeats. Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. SWISS: Q8IYU2 Gene ID: 57531 Database links: Entrez Gene: 57531 Human SwissProt: Q8IYU2 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: 293 cell lysates
Lane 2: 293T/17 cell lysates
Primary: Anti-HACE1 (bsm-51691M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 90-102 kD
Observed band size: 97 kD
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