產(chǎn)品編號(hào) | bsm-51646M |
英文名稱 | Mouse Anti-KANK1 antibody |
中文名稱 | KANK1單克隆抗體 |
別 名 | ANKRD 15; Ankyrin repeat domain 15; Ankyrin repeat domain containing protein 15; Ankyrin repeat domain-containing protein 15; DKFZp451G231; KANK; KANK1_HUMAN; KIAA0172; Kidney ankyrin repeat containing protein; Kidney ankyrin repeat-containing protein; KN motif and ankyrin repeat domain-containing protein 1; MGC43128. |
研究領(lǐng)域 | 腫瘤 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 新陳代謝 |
抗體來(lái)源 | Mouse |
克隆類型 | Monoclonal |
克 隆 號(hào) | Y6S1 |
交叉反應(yīng) | Human |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:25,IHC-F=1:25,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 65kDa |
細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | Recombinant human KANK1. |
亞 型 | IgG1,k |
純化方法 | affinity purified by Protein G |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 | |
產(chǎn)品圖片 |
Sample:
Lane 1: Jurkat cell lysates
Lane 2: K562 cell lysates
Primary: Anti-KANK1 (bsm-51646M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 150 kD
Paraformaldehyde-fixed, paraffin embedded (Human heart tissue sections); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KANK1) Monoclonal Antibody, Unconjugated (bsm-51646M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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