| 產(chǎn)品編號 | bsm-51605M |
| 英文名稱 | Mouse Anti-EEF1E1 antibody |
| 中文名稱 | 抑癌蛋白AIMP3單克隆抗體 |
| 別 名 | AIMP 3; AIMP3; Aminoacyl tRNA synthetase complex interacting multifunctional protein 3; Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3; ARS interacting multifunctional protein 3; Cyclin dependent kinase 6 inhibitor; Elongation factor p18; Eukaryotic translation elongation factor 1 epsilon 1; Eukaryotic translation elongation factor 1 epsilon-1; Homolog of rat elongation factor p18; MCA3_HUMAN; Multisynthase complex auxiliary component p18; Multisynthetase complex auxiliary component p18; p18; p18 component of aminoacyl tRNA synthetase complex; p18 INK4c; p18 NK6. |
| 研究領域 | 腫瘤 細胞生物 激酶和磷酸酶 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | G3E4 |
| 交叉反應 | Human,Mouse |
| 產(chǎn)品應用 | WB=1:500-4000,Flow-Cyt=1:25,ICC/IF=1:25
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 21kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human EEF1E1. |
| 亞 型 | IgG2b,κ |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2010] Function: Positive modulator of ATM response to DNA damage. Subunit: Component of the multisynthase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl and aspartyl-tRNA synthases, and three auxiliary proteins, EEF1E1/p18, AIMP2/p38 and AIMP1/p43. Interacts with ATM and ATR. The interaction with ATM, which takes place independently of TP53, is induced by DNA damage that may occur during genotoxic stress or cell growth. The interaction with ATR is enhanced by UV irradiation. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Down-regulated in various cancer tissues. Similarity: Contains 1 GST C-terminal domain. SWISS: O43324 Gene ID: 9521 Database links: Entrez Gene: 9521 Human Entrez Gene: 66143 Mouse SwissProt: O43324 Human SwissProt: Q9D1M4 Mouse |
| 產(chǎn)品圖片 |
Sample:
Lane 1: HepG2 cell lysates
Lane 2: Human testis tissue lysates
Lane 3: A549 cell lysates
Lane 4: A431 cell lysates
Lane 5: Jurkat cell lysates
Lane 6: Mouse brain tissue lysates
Primary: Anti-EEF1E1 (bsm-51605M) at 1/2000~4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 18 kD
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (EEF1E1) monoclonal Antibody, Unconjugated (bsm-51605M) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, Dylight? 554 Phalloidin (red) was used to stain the cell Cytoplasmic actin.The nuclear counter stain is DAPI (blue).
Blank control: Hela.
Primary Antibody (green line): Mouse Anti-EEF1E1 antibody (bsm-51605M)
Dilution: 1:25;
Isotype Control Antibody (blue line): Mouse IgG
Secondary Antibody : Goat anti-mouse IgG-AF488
Dilution: 1:400
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
