| 產品編號 | bsm-51593M |
| 英文名稱 | Mouse Anti-CBX8 antibody |
| 中文名稱 | 染色質結合蛋白Cbx8單克隆抗體 |
| 別 名 | Chromobox protein homolog 8; HPC3; Pc3; Polycomb 3 homolog; RC1; Rectachrome 1; CBX8_HUMAN. |
| 研究領域 | 染色質和核信號 表觀遺傳學 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | B2W3 |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-1000,Flow-Cyt=1:25
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 43kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CBX8 |
| 亞 型 | IgG1 |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Cbx8, also known as Pc3, is a member of the Polycomb gene family and is required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. Cbx8 contains a chromodomain and is able to modify chromatin and histones. Function: Component of a Polycomb group (PcG) multiproteinPRC1-like complex, a complex class required to maintain thetranscriptionally repressive state of many genes, including Hoxgenes, throughout development. PcG PRC1 complex acts via chromatinremodeling and modification of histones; it mediatesmonoubiquitination of histone H2A 'Lys-119', rendering chromatinheritably changed in its expressibility. Subunit: Component of a PRC1-like complex. Interacts with RING1RNF2, PCGF1, PCGF2, PCGF3, BMI1, PCGF5 AND PCGF6. Interacts withMLLT3 and histone H3. Interacts with PHC2. Subcellular Location: Nucleus. Similarity: Contains 1 chromo domain. SWISS: Q9HC52 Gene ID: 57332 Database links: Entrez Gene: 57332 Human SwissProt: Q9HC52 Human |
| 產品圖片 |
Sample:
Lane 1: 293 cell lysates
Lane 2: Hela cell lysates
Lane 3: A549 cell lysates
Primary: Anti-CBX8 (bsm-51593M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 78 kD
Blank control: A549.
Primary Antibody (green line): Mouse Anti-CBX8(C-term) antibody (bsm-51593M)
Dilution: 1:25;
Isotype Control Antibody (blue line): Mouse IgG
Secondary Antibody : Goat anti-mouse IgG-AF488
Dilution: 1:400
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
