| 產品編號 | bsm-51588M |
| 英文名稱 | Mouse Anti-USP5 antibody |
| 中文名稱 | 泛素羧基末端水解酶5單克隆抗體 |
| 別 名 | Deubiquitinating enzyme 5; Isopeptidase T; ISOT 1; ISOT; Ubiquitin carboxyl-terminal hydrolase 5; Ubiquitin isopeptidase T; Ubiquitin specific peptidase 5 (isopeptidase T); Ubiquitin specific peptidase 5; Ubiquitin specific processing protease 5; Ubiquitin specific protease 5 (isopeptidase T); Ubiquitin specific protease 5 (ubiquitin isopeptidase T); Ubiquitin thioesterase 5; Ubiquitin thiolesterase 5; Ubiquitin-specific-processing protease 5; UBP5_HUMAN. |
| 研究領域 | 細胞生物 表觀遺傳學 泛素 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | H4Y13 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 96kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human USP5. |
| 亞 型 | IgG1,Κ |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Profound changes in patterns of gene expression can result from relatively small changes in the concentrations of sequence specific transcription factors. Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010] Function: Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. Subunit: Interacts with TRIML1. Post-translational modifications: The N-terminus is blocked. The UniProt Consortium Similarity: Belongs to the peptidase C19 family. Contains 2 UBA domains. Contains 1 UBP-type zinc finger. SWISS: P45974 Gene ID: 8078 Database links: Entrez Gene: 8078 Human Entrez Gene: 22225 Mouse Omim: 601447 Human SwissProt: P45974 Human SwissProt: P56399 Mouse |
| 產品圖片 |
Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Kidney tissue lysates
Lane 3: Mouse Heart tissue lysates
Lane 4: Mouse Testis tissue lysates
Lane 5: Rat Cerebrum tissue lysates
Lane 6: Rat Testis tissue lysates
Lane 7: Human U251 cell lysates
Lane 8: Human HeLa cell lysates
Lane 9: Human Jurkat cell lysates
Lane 10: Human MCF-7 cell lysates
Primary: Anti-USP5 (bsm-51588M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 96 kDa
Observed band size: 103 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
