| 產(chǎn)品編號 | bsm-51462M |
| 英文名稱 | Mouse Anti-ATG12 antibody |
| 中文名稱 | 自噬相關(guān)蛋白12單克隆抗體 |
| 別 名 | Apg12; APG12-like; APG12L; ATG12; ATG12 autophagy related 12 homolog; Autophagy 12; Autophagy-related protein 12; HAPG12; Ubiquitin-like protein ATG12; ATG12_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細胞凋亡 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | AT03 |
| 交叉反應 | (predicted: Human) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human APG12L. |
| 亞 型 | IgG2b, Igk |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998[PubMed 9852036]).[supplied by OMIM]. Function: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. Subcellular Location: Cytoplasm. Tissue Specificity: Ubiquitous. Similarity: Belongs to the ATG12 family. SWISS: O94817 Gene ID: 9140 Database links: Entrez Gene: 9140 Human SwissProt: O94817 Human
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| 產(chǎn)品圖片 |
Sample:
Lane 1: ATG12 Recombinant protein
Primary:
Anti-ATG12 (bsm-51462M) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 15/21 kD
Observed band size: 24 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
