產(chǎn)品編號(hào) | bsm-51374M |
英文名稱 | Mouse Anti-PGK1 antibody |
中文名稱 | 磷酸激酶1抗體 |
別 名 | Cell migration-inducing gene 10 protein; MGC117307; MGC8947; MIG10; pgk1; PGK1_HUMAN; PGKA; Phosphoglycerate kinase 1; Primer recognition protein 2; PRP 2; PRP 2. |
研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 激酶和磷酸酶 新陳代謝 |
抗體來源 | Mouse |
克隆類型 | Monoclonal |
克 隆 號(hào) | 6C1 |
交叉反應(yīng) | Human,Mouse |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 44kDa |
細(xì)胞定位 | 細(xì)胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human PGK1: 1-417/417 |
亞 型 | IgG2a,k |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq] Function: In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). Subunit: Monomer. Subcellular Location: Cytoplasm. DISEASE: Phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]: A condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the phosphoglycerate kinase family. SWISS: P00558 Gene ID: 5230 Database links: Entrez Gene: 5230 Human Entrez Gene: 18655 Mouse Omim: 311800 Human SwissProt: P00558 Human SwissProt: P09411 Mouse Unigene: 78771 Human Unigene: 336204 Mouse Unigene: 336205 Mouse Unigene: 108127 Rat |
產(chǎn)品圖片 |
Sample:
Lane 1: Mouse NIH/3T3 cell lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Heart tissue lysates
Lane 4: Mouse Kidney tissue lysates
Lane 5: Mouse Testis tissue lysates
Lane 6: Mouse Liver tissue lysates
Lane 7: Human HeLa cell lysates
Lane 8: Human A431 cell lysates
Lane 9: Human MOLT4 cell lysates
Primary: Anti-PGK1 (bsm-51374M) at 1/1000 dilution
Secondary: Alexa Fluor 790 AffiniPure Goat Anti-Mouse IgG, light chain specific
Predicted band size: 44 kDa
Observed band size: 44 kDa
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