| 產(chǎn)品編號 | Abk002 |
| 英文名稱 | Rabbit Anti-Neural Lineage Markers Antibody Assembly kit antibody |
| 中文名稱 | 神經(jīng)譜系標記抗體套裝 |
| 別 名 | Neural Lineage Markers Antibody Assembly kit(TUBB3,GFAP,MAP2,Nestin,NeuN,Vimentin) 神經(jīng)譜系標記抗體套裝,神經(jīng)系表面標記抗體套裝(TUBB3,GFAP,MAP2,Nestin,NeuN,Vimentin) |
| 研究領(lǐng)域 | 神經(jīng)生物學(xué) 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
In central nervous system, neural stem cells (NSC) differentiate into multiple neural lineages, including neural precursors, astrocytes, neurons, and oligodendrocytes. Neuronal lineage markers are endogenous tags that are expressed in different cells along neurogenesis and differentiated cells such as neurons. We can use it to detect and identify different types of cells by different techniques. Neural Lineage Markers Antibody Assembly kit is one economical package of selected antibodies for neural lineage markers, including neural stem cell and progenitor marker Nestin, mature neuron marker NeuN and MAP2, Immature neuron maker TUBB3, radial glia cell marker Vimentin, mature astrocytes and other glial cell marker GFAP. These antibodies have been tested in a variety of applications and species. For specific application information of different antibodies, please refer to each individual antibody datasheet. Neural Lineage Markers Antibody Assembly kit Rabbit Anti-TUBB3 (Neuronal Marker) antibody (bs-4512R) Rabbit Anti-GFAP antibody (bs-0199R) Rabbit Anti-MAP2 antibody (bs-1369R) Rabbit Anti-Nestin antibody (bs-0008R) Rabbit Anti-NeuN antibody (bs-1613R) Rabbit Anti-Vimentin antibody (bs-0756R) SWISS: N/A Gene ID: N/A Database links: Neural Lineage Markers Antibody Assembly kit Rabbit Anti-TUBB3 (Neuronal Marker) antibody (bs-4512R) Rabbit Anti-GFAP antibody (bs-0199R) Rabbit Anti-MAP2 antibody (bs-1369R) Rabbit Anti-Nestin antibody (bs-0008R) Rabbit Anti-NeuN antibody (bs-1613R) Rabbit Anti-Vimentin antibody (bs-0756R)
在中樞神經(jīng)系統(tǒng)中，神經(jīng)干細胞(NSC)可分化為多種神經(jīng)細胞類型，包括神經(jīng)前體、星形膠質(zhì)細胞、神經(jīng)元和少突膠質(zhì)細胞等。神經(jīng)譜系標記是一些在神經(jīng)發(fā)生或分化后不同細胞類型中特征性表達的一些蛋白。我們利用可以這些特征性標記通過不同的技術(shù)來檢測和識別不同類型的細胞。神經(jīng)譜系標記抗體裝配試劑盒極具性價比，包含了最具代表性的神經(jīng)譜系標記抗體，包括神經(jīng)干細胞和祖細胞標記Nestin、成熟神經(jīng)元標記NeuN和MAP2、未成熟神經(jīng)元標記TUBB3、放射狀膠質(zhì)細胞標記Vimentin、成熟星形膠質(zhì)細胞和其他膠質(zhì)細胞標記GFAP。這些抗體在多種應(yīng)用和物種中進行了測試。有關(guān)抗體具體使用信息，請參閱各個抗體產(chǎn)品數(shù)據(jù)表。 |
| 產(chǎn)品圖片 |
Primary: Anti-MAP2 (bs-1369R)
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP2) Polyclonal Antibody, Unconjugated (bs-1369R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Primary: Anti-TUBB3 (Neuronal Marker) (bs-4512R)
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TUBB3/beta III Tubulin(Neuronal Marker) Polyclonal Antibody, Unconjugated(bs-4512R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Primary: Anti- GFAP (bs-0199R)
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GFAP Polyclonal Antibody, Unconjugated(bs-0199R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Primary:Anti-NeuN(bs-1613R)
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Primary: Anti- Vimentin (bs-0756R)
Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vimentin) Polyclonal Antibody, Unconjugated (bs-0756R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Primary: Anti-Nestin Polyclonal Antibody, Unconjugated(bs-0008R)
Primary: Anti-MAP2 (bs-1369R)
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP2) Polyclonal Antibody, Unconjugated (bs-1369R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Primary: Anti- GFAP (bs-0199R)
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4°C, followed by a conjugated secondary (bs-0295G-Cy3) at [1:500] for 90 minutes and DAPI staining of the nuclei.
Primary: Anti-Nestin Polyclonal Antibody, Unconjugated(bs-0008R)
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min;
Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nestin Polyclonal Antibody, Unconjugated(bs-0008R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
