產(chǎn)品編號 | bsk31002 |
英文名稱 | Human LH ELISA Kit |
中文名稱 | 人促黃體生成素酶聯(lián)免疫試劑盒 |
種 屬 | Human |
線性范圍 | 0-200mIU/ml |
應用范圍 | 人血清 |
檢測限 | 2mIU/ml |
適用樣品基質 | 人血清 |
保存條件 | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.). |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
產(chǎn)品介紹 |
This gene is a member of the glycoprotein hormone beta chain family and encodes the beta subunit of luteinizing hormone (LH). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.3. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism. 人促黃體生成激素(hLH)是由垂體前葉分泌的糖蛋白激素,其分子量大約為30,000,由兩條多肽鏈(即α和β亞單位)組成。LH的α亞單位由89個氨基酸殘基組成,并與FSH、TSH和 hCG 的α亞單位的結構相同,β亞單位在上述激素之間是不同的,從而賦予各激素各自的生物及免疫學特性。 在女性體中,LH在卵泡期與FSH一起促進卵泡的成熟、雌性激素的合成和分泌;促進排卵和使排卵后的卵泡轉變?yōu)辄S體。促進間質的生長。并促進黃體合成和孕激素與雌性激素的分泌。男性LH促進睪丸間質細胞增生,促進其合成和分泌睪酮。由于LH和FSH的作用是互相協(xié)同的,故二者常同時測定。 LH增高見于Klindfelter綜合癥,Turner綜合癥,性腺切除后及促性腺激素產(chǎn)生腫瘤;減低見于西蒙氏病,席漢氏綜合癥,肥胖性生殖器退化綜合癥,垂體性侏儒,睪丸腫瘤,卵巢腫瘤,神經(jīng)性厭食及使用雌激素后。 |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |