| 產(chǎn)品編號(hào) | bsm-33442M |
| 英文名稱 | Mouse Anti-lamin B1 antibody |
| 中文名稱 | 核纖層蛋白B（核內(nèi)參）單克隆抗體 |
| 別 名 | LMNB1_HUMAN; Lamin-B1; LMNB1; LMN2; LMNB; LMN; ADLD; MCPH26; |
| 研究領(lǐng)域 | 細(xì)胞生物 染色質(zhì)和核信號(hào) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 |
| 抗體來(lái)源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號(hào) | 7B7 |
| 交叉反應(yīng) | Human,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 64kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant Protein human Lamin B1 Protein |
| 亞 型 | IgG1 |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010]. Function: Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin Subunit: Homodimer. Interacts with lamin-associated polypeptides IA, IB and 2. Subcellular Location: Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side Post-translational modifications: B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. DISEASE: Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis. Similarity: Belongs to the intermediate filament family. SWISS: P20700 Gene ID: 4001 Database links: Entrez Gene: 396223 Chicken Entrez Gene: 4001 Human Entrez Gene: 16906 Mouse Omim: 150340 Human SwissProt: P14731 Chicken SwissProt: P20700 Human SwissProt: P14733 Mouse Unigene: 89497 Human Unigene: 4105 Mouse Unigene: 11362 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human Molt-4 cell lysates
Lane 2: Human Hela cell lysates
Lane 3: Human U-2os cell ysates
Primary: Anti-Lamin B (bsm-33442M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1? Monoclonal Antibody, Unconjugated(bsm-33442M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1? Monoclonal Antibody, Unconjugated(bsm-33442M) at 1:300 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1? Monoclonal Antibody, Unconjugated(bsm-33442M) at 1:300 overnight at 4°C, followed by conjugation to the bs-0296G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1? Monoclonal Antibody, Unconjugated(bsm-33442M) at 1:300 overnight at 4°C, followed by conjugation to the bs-0296G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1? Monoclonal Antibody, Unconjugated(bsm-33442M) at 1:300 overnight at 4°C, followed by conjugation to the bs-0296G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1? Monoclonal Antibody, Unconjugated(bsm-33442M) at 1:300 overnight at 4°C, followed by conjugation to the bs-0296G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1? Monoclonal Antibody, Unconjugated(bsm-33442M) at 1:300 overnight at 4°C, followed by conjugation to the bs-0296G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded (human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Lamin B (Nuclear Loading Control) ) Monoclonal Antibody, Unconjugated (bsm-33442M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Lamin B (Nuclear Loading Control) ) Monoclonal Antibody, Unconjugated (bsm-33442M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Lamin B (Nuclear Loading Control) ) Monoclonal Antibody, Unconjugated (bsm-33442M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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