| 產(chǎn)品編號(hào) | bs-21564R |
| 英文名稱 | Rabbit Anti-Glutathione Reductase antibody |
| 中文名稱 | 谷胱甘肽還原酶抗體 |
| 別 名 | glutathione reductase; GLUR; Glutathione reductase mitochondrial; GR; Gr1; GRase; GRD 1; GRD1; GSR; MGC78522; GSHR_HUMAN. |
| 研究領(lǐng)域 | 免疫學(xué) 激酶和磷酸酶 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 57kDa |
| 細(xì)胞定位 | 分泌型蛋白 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Glutathione Reductase : 361-460/500 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Glutathione reductase (GR) is a member of pyridine nucleotide- disulfideoxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione (GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+?2GSH + NADP+. In mosteukaryotic cells, GR maintains the ratio of [GSH]/[GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis. Function: Maintains high levels of reduced glutathione in the cytosol. Subunit: Homodimer; disulfide-linked. Subcellular Location: Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm. Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. SWISS: P47791 Gene ID: 14782 Database links: Entrez Gene: 2936 Human Entrez Gene: 14782 Mouse Omim: 138300 Human SwissProt: P00390 Human SwissProt: P47791 Mouse Unigene: 271510 Human Unigene: 283573 Mouse Unigene: 19721 Rat 胱甘肽還原酶為一種黃素酶，可催化氧化型谷胱甘肽轉(zhuǎn)變成還原型谷胱甘肽(GSH)，從而提高細(xì)胞液中GSH的含量。 GSH具有抵抗自由基氧化對(duì)機(jī)體損傷的作用，GSH的重要生理功能在于能還原體內(nèi)生成的過氧化氫(H2O2)。GSH的儲(chǔ)量與創(chuàng)傷、腫瘤等的預(yù)后，以及人體衰老有關(guān)。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glutathione Reductase) Polyclonal Antibody, Unconjugated (bs-21564R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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