| 產(chǎn)品編號 | bs-21514R |
| 英文名稱 | Rabbit Anti-PPAR delta + beta antibody |
| 中文名稱 | D型-過氧化酶活化增生受體抗體 |
| 別 名 | PPAR delta+beta; FAAR; MGC3931; NR1C2; NUC1; NUCI; NUCII; Nuclear hormone receptor 1; Nuclear receptor subfamily 1 group C member 2; Peroxisome proliferative activated receptor delta; Peroxisome proliferator-activated receptor beta (PPAR-beta); Peroxisome proliferator-activated receptor beta; Peroxisome proliferator-activated receptor delta; PPAR beta; PPAR-beta; PPAR-delta; PPAR-? PPARB; PPARD; PPARD_HUMAN. |
| 研究領(lǐng)域 | 心血管 免疫學(xué) 細胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse PPAR delta + beta : 1-100/440 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Heterodimer with the retinoid X receptor. Subcellular located at nuclear Tissue specificity: Heart, adrenal and intestine. Belongs to the nuclear hormone receptor family. NR1 subfamily. It Contains 1 nuclear receptor DNA-binding domain. Function: Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. Subcellular Location: Nucleus. Tissue Specificity: Ubiquitous with maximal levels in placenta and skeletal muscle. Similarity: Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain. SWISS: P35396 Gene ID: 19015 Database links: Entrez Gene: 5467 Human Entrez Gene: 19015 Mouse Omim: 600409 Human SwissProt: Q03181 Human SwissProt: P35396 Mouse Unigene: 696032 Human Unigene: 328914 Mouse Unigene: 96181 Rat 類固醇受體（Steroid Receptors） 研究人員指出該分子的功能，極可能就是阻斷心血管免疫發(fā)炎反應(yīng)的訊號傳遞過程。PPAR－delta基因是控制肌肉發(fā)展的重要基因，在增加老鼠耐力的同時還能幫助它燃燒掉脂肪，研究人員發(fā)現(xiàn)，對于與新陳代謝有關(guān)的各項疾病，從心臟病到肥胖癥都有啟發(fā)性的意義，他可以阻止脂肪沉淀在動脈壁上，進而防止動脈硬化癥的發(fā)生,該蛋白參與脂肪代謝、肥胖、糖尿病、動脈硬化和癌癥的發(fā)病。 在動脈硬化現(xiàn)象發(fā)生的早期，免疫細胞會促使血管慢性的發(fā)炎，因而造成血管不斷地吸收和輸送脂肪，進而導(dǎo)致脂肪的堆積與血管硬化，而PPAR-delta就像警衛(wèi)分子一樣，時時降低發(fā)炎反應(yīng)和抑制動脈硬化斑的形成，因此該分子應(yīng)該是相關(guān)藥物研發(fā)，相當(dāng)值得切入的目標。 研究人員認為，這個發(fā)現(xiàn)將給藥廠研發(fā)治療心血管疾病的新藥提供新的線索。 |
| 產(chǎn)品圖片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-PPAR delta + beta (bs-21514R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPAR delta + beta) Polyclonal Antibody, Unconjugated (bs-21514R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
