| 產(chǎn)品編號 | bs-23424R |
| 英文名稱 | Rabbit Anti-AKAP14 antibody |
| 中文名稱 | A激酶錨定蛋白14抗體 |
| 別 名 | A kinase (PRKA) anchor protein 14; A kinase anchor protein 14; PRKA14; AKAP-14; AKA14_RAT; Protein kinase A-anchoring protein 14; Protein kinase A anchoring protein 14. TAKAP-1.2; Testis-specific A-kinase-anchoring protein TAKAP-80. |
| 研究領(lǐng)域 | 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat AKAP14: 81-180/502 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. The human ortholog of this gene has been characterized. It is reported to be enriched in airway cilia and to regulate ciliary beat frequency by anchoring the protein kinase A holoenzyme near substrates in the axoneme. [provided by RefSeq, May 2013]. Function: Binds to type II regulatory subunits of protein kinase A and anchors/targets them. Subunit: Binds to type II regulatory subunits (RII). Subcellular Location: Cytoplasm. Tissue Specificity: Present in cilia (at protein level). Expressed in tissues containing axoneme-based organelles (cilia and/or flagella): trachea and testis. Highly expressed in airway cilia. SWISS: O35817 Gene ID: 60332 Database links: Entrez Gene: 434756 Mouse Unigene: 471848 Mouse Unigene: 42933 Rat |
| 產(chǎn)品圖片 |
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-AKAP14 (bs-23424R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
