| 產(chǎn)品編號 | bsm-33396M |
| 英文名稱 | Mouse Anti-ATG7/APG7 antibody |
| 中文名稱 | 自噬相關(guān)蛋白7單克隆抗體 |
| 別 名 | Apg 7; ATG7; APG7 autophagy 7 like; APG7 autophagy 7-like (S. cerevisiae); APG7 like; APG7, S. cerevisiae, homolog of; APG7L; ATG 7; atg7; ATG7 autophagy related 7 homolog (S. cerevisiae); ATG7 autophagy related 7 homolog; Atg7l; Autophagy 7, S. cerevisiae, homolog of; Autophagy related protein 7; Autophagy-related 7 (yeast); DKFZp434N0735; GSA 7; GSA7; hAGP7; Ubiquitin activating enzyme E1 like protein; ATG7_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 細胞凋亡 細胞自噬 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 5E6 |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 78kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human ATG7 Protein: 71-333/703 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq, Jan 2009]. Function: Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3. Subunit: Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Subcellular Location: Cytoplasm (Probable). Tissue Specificity: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. Similarity: Belongs to the ATG7 family. SWISS: O95352 Gene ID: 10533 Database links: Entrez Gene: 10533 Human Entrez Gene: 415961 Chicken Entrez Gene: 74244 Mouse Omim: 608760 Human SwissProt: Q5ZKY2 Chicken SwissProt: O95352 Human SwissProt: Q9D906 Mouse Unigene: 38032 Human Unigene: 275332 Mouse Unigene: 162765 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG7) Monoclonal Antibody, Unconjugated (bsm-33396M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
