| 產(chǎn)品編號 | bs-14026R |
| 英文名稱 | Rabbit Anti-Synaptopodin antibody |
| 中文名稱 | 突觸足蛋白抗體 |
| 別 名 | 9030217H17RIK; 9130229N11; 9330140I15Rik; AW046661; SYNAPTOPODIN; KIAA1029; SYNPO; SYNPO_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Rabbit,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 100kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Synaptopodin: 681-780/929 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Synaptopodin is an actin-associated protein that may play a role in actin-based cell shape and motility. May be essential for the formation of spine apparatuses in spines of telencephalic neurons, involved in synaptic plasticity. The name synaptopodin derives from the protein's associations with postsynaptic densities and dendritic spines and with renal podocytes. Subunit: Interacts with BAIAP1. Interacts with actin. Interacts (via PPxY motifs) with WWC1 (via WW domains). Subcellular Location: Cytoplasm, cytoskeleton. Cell junction, tight junction. Perikaryon. Cell projection, dendritic spine. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Note=Localized at the tight junction of cells. In brain, localized to the postsynaptic densities and in the perikarya. Asssociated with dendritic spines of a subset of synapses. Tissue Specificity: Expressed in brain, namely in the olfactory bulb, cerebral cortex, striatum, and hippocampus, but not in the cerebellum. Also expressed in the podocytes of kidney glomeruli. In the hippocampus, mainly expressed in the principal cell layer of the dentate gyrus and Ammon's horn. Similarity: Belongs to the synaptopodin family. SWISS: Q8N3V7 Gene ID: 11346 Database links: Entrez Gene: 11346 Human Entrez Gene: 104027 Mouse Omim: 608155 Human SwissProt: Q8N3V7 Human SwissProt: Q8CC35 Mouse Unigene: 435228 Human synaptopodin是一種與肌動蛋白微絲偶聯(lián)的蛋白，其富含脯氨酸,為線狀蛋白質(zhì)，在動物體內(nèi)表達(dá)于腎小球的足細(xì)胞和后腦的突觸內(nèi)，synaptopodin被認(rèn)為是足細(xì)胞特異的分化成熟標(biāo)志物。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Synaptopodin) Polyclonal Antibody, Unconjugated (bs-14026R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control (blue line): U-87MG (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti-Synaptopodin antibody (bs-3152R), Dilution: 0.2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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