| 產(chǎn)品編號(hào) | bs-20824R |
| 英文名稱 | Rabbit Anti-CK5+CK6 antibody |
| 中文名稱 | 細(xì)胞角蛋白5+6抗體 |
| 別 名 | CK5+6; Cytokeratin 5 + 6; Cytokeratin 5; Keratin, type II cytoskeletal 5; CK-5; Keratin-5; K5; 58 kDa cytokeratin; KRT5; CK 5; DDD; EBS 2; EBS2; K5; Keratin 5 (epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types); Keratin 5; Keratin Type II Cytoskeletal 5; Keratin5; KRT 5; KRT 5A; KRT5.KRT6A; CK6A; CK6B; CK6C; CK6D; CK 6E; K6A; K6C; K6D; KRT6C; Cytokeratin 6a; Cytokeratin 6B; Cytokeratin 6C; Cytokeratin 6D; Cytokeratin 6E; Cytokeratin6; k6a keratin; K6B; K6b keratin; K6c keratin; K6d keratin; K6e keratin; k6hf; Keratin 6 isoform K6e; Keratin 6C; Keratin 6E; Keratin K6h; Keratin type II cytoskeletal 6A; Keratin type II cytoskeletal 6B; Keratin type II cytoskeletal 6C; Keratin type II cytoskeletal 6D; Keratin type II cytoskeletal 6E; KRT6; KRT6A; KRT6B; KRT6C; KRT6D; KRT6E; MGC102925. |
| 研究領(lǐng)域 | 腫瘤 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CK5+CK6: 161-220/590 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Keratins 5, 6 are members of type II keratins family that are specifically expressed in the inner root sheath of hair follicles. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Keratin 18 is memeber of the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. SWISS: P02538 Gene ID: 3852 Database links: Entrez Gene: 3852 Human Entrez Gene: 3853 Human Entrez Gene: 3854 Human Omim: 148040 Human SwissProt: P02538 Human SwissProt: P04259 Human SwissProt: P13647 Human 細(xì)胞角蛋白5&6是高分子量細(xì)胞角蛋白，分子量分別為58KDa和56KDa。其表達(dá)在皮膚的基底細(xì)胞、棘層細(xì)胞和部分前列腺基底細(xì)胞。主要用于間皮瘤和腺癌的鑒別診斷，并可輔助診斷前列腺良惡性病變。 |
| 產(chǎn)品圖片 |
Sample:
Esophagus (Mouse) Lysate at 40 ug
Primary: Anti-CK5+CK6 (bs-20824R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62/60 kD
Observed band size: 62/60 kD
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-CK5+CK6 (bs-20824R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62/60 kD
Observed band size: 62/60 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK5+CK6) Polyclonal Antibody, Unconjugated (bs-20824R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK5+CK6) Polyclonal Antibody, Unconjugated (bs-20824R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control (blue line): Hela (fixed with 70% ethanol (Overmight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min at -20℃).
Primary Antibody (green line): Rabbit Anti-CK5+CK6 antibody (bs-20824R), Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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