| 產(chǎn)品編號 | bs-12679R |
| 英文名稱 | Rabbit Anti-VAC14 antibody |
| 中文名稱 | VAC14蛋白抗體 |
| 別 名 | ArPIKfyve; FLJ10305; FLJ36622; FLJ46582; MGC149815; MGC149816; OTTHUMP00000174908; Protein VAC14 homolog; Tax1 (human T cell leukemia virus type I) binding protein 1; Tax1 (human T cell leukemia virus type I) binding protein 2; Tax1 binding protein 2; Tax1-binding protein 2; TAX1BP2; TRX; VAC 14; vac14; Vac14 homolog (S. cerevisiae); Vac14 homolog; VAC14_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) 淋巴細(xì)胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Zebrafish,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 88kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human VAC14: 1-100/782 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The content of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) in endosomal membranes changes dynamically with fission and fusion events that generate or absorb intracellular transport vesicles. VAC14 is a component of a trimolecular complex that tightly regulates the level of PtdIns(3,5)P2. Other components of this complex are the PtdIns(3,5)P2-synthesizing enzyme PIKFYVE (MIM 609414) and the PtdIns(3,5)P2 phosphatase FIG4 (MIM 609390). VAC14 functions as an activator of PIKFYVE (Sbrissa et al., 2007 [PubMed 17556371]).[supplied by OMIM, Feb 2010] Function: The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2). Acts as a positive activator of PIKfyve kinase activity. Also required to maintain normal levels of phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 5-phosphate (PtdIns(5)P). Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes. Subcellular Location: Endosome membrane. Microsome membrane. Mainly associated with membranes of the late endocytic pathway. Tissue Specificity: Ubiquitously expressed. Similarity: Belongs to the VAC14 family. Contains 6 HEAT repeats. SWISS: Q08AM6 Gene ID: 55697 Database links: Entrez Gene: 55697 Human Entrez Gene: 234729 Mouse Omim: 604632 Human SwissProt: Q08AM6 Human SwissProt: Q80WQ2 Mouse Unigene: 445061 Human Unigene: 274894 Mouse Unigene: 46366 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VAC14) Polyclonal Antibody, Unconjugated (bs-12679R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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