| 產(chǎn)品編號 | bs-12665R |
| 英文名稱 | Rabbit Anti-TMEM85 antibody |
| 中文名稱 | 跨膜蛋白85抗體 |
| 別 名 | Cell proliferation-inducing gene 17 protein; EMC4; ER membrane protein complex subunit 4; HSPC184; PIG17; tmem85; TMM85_HUMAN; Transmembrane protein 85. |
| 研究領(lǐng)域 | 細胞生物 免疫學 跨膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rabbit,Pig,Sheep,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TMEM85: 2-100/183 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
May mediate anti-apoptotic activity. Subcellular Location: Membrane. Tissue Specificity: Isoform 1 is expressed in brain and heart. Isoform 2 is expressed in heart. Similarity: Belongs to the TMEM85 family. SWISS: Q5J8M3 Gene ID: 51234 Database links: Entrez Gene: 51234 Human Entrez Gene: 68032 Mouse SwissProt: Q5J8M3 Human SwissProt: Q9CZX9 Mouse Unigene: 250905 Human Unigene: 220942 Mouse Unigene: 219228 Rat |
| 產(chǎn)品圖片 |
Sample:
U251(Human) Cell Lysate at 40 ug
Primary: Anti-TMEM85(bs-12665R) at 1/500 dilution
Anti-TMEM85(bs-12665R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 17 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEM85) Polyclonal Antibody, Unconjugated (bs-12665R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEM85) Polyclonal Antibody, Unconjugated (bs-12665R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
