| 產(chǎn)品編號 | bs-19934R |
| 英文名稱 | Rabbit Anti-TINP1/NSA2 antibody |
| 中文名稱 | 轉(zhuǎn)移生長因子誘導核蛋白1抗體 |
| 別 名 | HCLG1; TINP1; CDK105; HCL-G1; HUSSY29; HUSSY-29; Hairy cell leukemia protein 1; NSA2; NSA2_HUMAN; Ribosome biogenesis protein NSA2 homolog; TGF beta inducible nuclear protein 1; TGF-beta-inducible nuclear protein 1. |
| 研究領域 | 細胞生物 細胞周期蛋白 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 30kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TINP1/NSA2: 1-100/260 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a nucleolar protein involved in cell cycle regulation and proliferation. This gene was identified based on sequence similarity to a highly conserved Saccharomyces cerevisiae gene encoding a pre-ribosomal protein, which is involved in large ribosomal subunit biogenesis. The encoded protein is found at elevated levels in diabetic nephropathy. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified. [provided by RefSeq, Nov 2012] Function: Involved in the biogenesis of the 60S ribosomal subunit. May play a part in the quality control of pre-60S particles. Subunit: Component of the pre-66S ribosomal particle. Subcellular Location: Nucleus > nucleolus. Similarity: Belongs to the ribosomal protein S8e family. SWISS: O95478 Gene ID: 10412 Database links: Entrez Gene: 10412 Human Entrez Gene: 433230 Mouse Entrez Gene: 59050 Mouse Entrez Gene: 636306 Mouse SwissProt: O95478 Human SwissProt: Q9CR47 Mouse Unigene: 482526 Human Unigene: 390634 Mouse Unigene: 422907 Mouse Unigene: 129634 Rat Unigene: 15195 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TINP1/NSA2) Polyclonal Antibody, Unconjugated (bs-19934R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TINP1/NSA2) Polyclonal Antibody, Unconjugated (bs-19934R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
