| 產品編號 | bs-20013R |
| 英文名稱 | Rabbit Anti-ALPI antibody |
| 中文名稱 | 堿性磷酸酶(腸型)抗體 |
| 別 名 | PPBI_HUMAN; Intestinal-type alkaline phosphatase; EC:3.1.3.1; IAP; Intestinal alkaline phosphatase; alkaline phosphatase, intestinal; 腸道堿性磷酸酶; 腸堿性磷酸酶; 堿性磷酸酶，腸型; 腸型堿性磷酸酶; |
| 研究領域 | 腫瘤 免疫學 信號轉導 激酶和磷酸酶 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 53kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ALPI: 351-450/528 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation. [provided by RefSeq, Jul 2008] Subunit: Homodimer. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Similarity: Belongs to the alkaline phosphatase family. SWISS: P09923 Gene ID: 248 Database links: Entrez Gene: 248 Human Omim: 171740 Human SwissProt: P09923 Human Unigene: 284255 Human Unigene: 37009 Human |
| 產品圖片 |
Sample:
Lane 1: Human LOVO cell lysates
Lane 2: Human FHC cell lysates
Lane 3: Human SW480 cell lysates
Primary: Anti-ALPI (bs-20013R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 65 kDa
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
