| 產(chǎn)品編號 | bs-18474R |
| 英文名稱 | Rabbit Anti-ZDHHC13/HIP14L antibody |
| 中文名稱 | ZDHHC13蛋白抗體 |
| 別 名 | DHHC 13; DHHC-13; FLJ10852; FLJ10941; HIP14 like; HIP14 related protein; HIP14-related protein; HIP14L; HIP3RP; Huntingtin interacting protein 14 related protein; Huntingtin interacting protein HIP3RP; Huntingtin-interacting protein 14-related protein; Huntingtin-interacting protein HIP3RP; Hypothetical protein similar to ankyrin repeat containing priotein AKR1; MGC64994; Palmitoyltransferase ZDHHC13; Probable palmitoyltransferase ZDHHC13; Putative MAPK activating protein PM03; Putative MAPK-activating protein PM03; Putative NF kappa B activating protein 209; Putative NF-kappa-B-activating protein 209; ZDH13_HUMAN; ZDHHC 13; ZDHHC13; Zinc finger DHHC domain containing 13; Zinc finger DHHC domain-containing protein 13. |
| 研究領(lǐng)域 | 細胞生物 染色質(zhì)和核信號 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 71kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZDHHC13/HIP14L: 501-600/622 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ZDHHC13 is a 622 amino acid multi-pass membrane protein that contains one DHHC-type zinc finger and six ANK repeats. Expressed as multiple alternatively spliced isoforms, HIP14L functions as a palmitoyltransferase that catalyzes the conversion of palmitoyl-CoA and a protein-cyeteine to an S-palmitoyl protein and CoA and, via this catalytic activity, may be involved in the NFⅹB signaling pathway. The gene encoding HIP14L maps to human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11. Function: Palmitoyltransferase for HD and GAD2 (By similarity). Mediates Mg(2+) transport. Subcellular Location: Golgi apparatus membrane. Cytoplasmic vesicle membrane. Low extracellular Mg(2+) induces increase in Golgi and in post-Golgi vesicles. Similarity: Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily. Contains 6 ANK repeats. Contains 1 DHHC-type zinc finger. SWISS: Q8IUH4 Gene ID: 54503 Database links: Entrez Gene: 422980 Chicken Entrez Gene: 54503 Human Entrez Gene: 243983 Mouse Entrez Gene: 494107 Zebrafish Omim: 612815 Human SwissProt: Q8IUH4 Human SwissProt: Q9CWU2 Mouse Unigene: 188569 Human Unigene: 279116 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZDHHC13) Polyclonal Antibody, Unconjugated (bs-18474R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZDHHC13) Polyclonal Antibody, Unconjugated (bs-18474R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
