| 產(chǎn)品編號(hào) | bs-18228R |
| 英文名稱 | Rabbit Anti-PGAM5 antibody |
| 中文名稱 | 絲氨酸/蘇氨酸蛋白磷酸酶PGAM5抗體 |
| 別 名 | Bcl-XL-binding protein v68; BXLBv68; MGC5352; mitochondrial; PGAM5; PGAM5_HUMAN; Phosphoglycerate mutase family member 5; Serine/threonine protein phosphatase PGAM5 mitochondrial; Serine/threonine-protein phosphatase PGAM5. |
| 研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 激酶和磷酸酶 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 32kDa |
| 細(xì)胞定位 | 細(xì)胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PGAM5: 51-150/289 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Members of the PGAM (phosphoglycerate mutase) family of proteins are important components of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism. They are responsible for catalyzing the transfer of phospho groups between the carbon atoms of phosphoglycerates. PGAM5 (phosphoglycerate mutase family member 5), also known as Bcl-xL-binding protein v68, is a 289 amino acid protein belonging to the BPG-dependent PGAM subfamily. PGAM5 exists as two isoforms produced by alternative splicing events, with isoform two localized to the cytoplasm and isoform one localized to both the cytoplasm and the nucleus. PGAM5 forms a dimer and has been found to interact with Bcl-xS/L and Keap1. Function: Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression. Subcellular Location: Mitochondrion. Mitochondrion outer membrane. Membrane. Isoform 2 overexpression results in the formation of disconnected punctuate mitochondria distributed throughout the cytoplasm. Isoform 1 overexpression results in the clustering of mitochondria around the nucleus. Similarity: Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily. SWISS: Q96HS1 Gene ID: 192111 Database links: Entrez Gene: 192111 Human Entrez Gene: 72542 Mouse SwissProt: Q96HS1 Human SwissProt: Q8BX10 Mouse Unigene: 102558 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PGAM5) Polyclonal Antibody, Unconjugated (bs-18228R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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