| 產(chǎn)品編號 | bs-17863R |
| 英文名稱 | Rabbit Anti-MSTO1 antibody |
| 中文名稱 | MSTO1蛋白抗體 |
| 別 名 | DKFZp686B1757; DKFZp686I01261; FLJ10504; LST005; misato homolog 1 (Drosophila); MST; Protein misato homolog 1; RP11 29H23.3. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 信號轉(zhuǎn)導 新陳代謝 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 62kDa |
| 細胞定位 | 細胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MSTO1: 151-250/570 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Drosophila melanogaster is a proven and effective model for studying developmental and cellular processes common to higher eukaryotes. Approximately 13,600 genes have been elucidated from more than 120 megabases of euchromatin, and they are organized among the chromosomes 2, 3, 4, X and Y, with the Y chromosome being predominately heterochromatic (1). Drosophila genes can be categorized based on the type of protein for which they encode and are represented by six major classifications, which include intracellular signaling proteins, transmembrane proteins, RNA binding proteins, secreted factors, transcription regulators (basic helix-loop-helix, homeodomain containing, zinc finger containing, and chromatin associated) or other functional proteins (2). The misato gene encodes a protein that contains a mixture of peptide motifs found in alpha-, beta-, and gamma-tubulins, as well as a motif related to part of the myosin heavy chain proteins (3-5). Null mutations at the misato locus of Drosophila melanogaster are associated with irregular chromosomal segregation at cell division and result in larvae that have reduced levels of imaginal disk tissue, a reduction in brain size, and die during the larval stage of development (4,5). Function: MSTO1, or misato homolog 1 (Drosophila), is involved in the regulation of mitochondrial distribution and morphology. Subcellular Location: Mitochondrion outer membrane. Tissue Specificity: Present in all cell lines tested (at protein level). Widely expressed. Similarity: Belongs to the misato family. SWISS: Q9BUK6 Gene ID: 55154 Database links: Entrez Gene: 55154 Human GenBank: NM_018116 Human SwissProt: Q9BUK6 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MSTO1) Polyclonal Antibody, Unconjugated (bs-17863R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
