產(chǎn)品編號 | bs-17836R |
英文名稱 | Rabbit Anti-MRS2 antibody |
中文名稱 | MRS2蛋白抗體 |
別 名 | Gm902; HPT; Magnesium transporter MRS2 homolog, mitochondrial; MGC78523; MRS2 like protein; MRS2 like, magnesium homeostasis factor; MRS2 like, magnesium homeostasis factor (S. cerevisiae); MRS2 magnesium homeostasis factor homolog (S. cerevisiae); MRS2L; RPT |
研究領(lǐng)域 | 腫瘤 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) 新陳代謝 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Cat,Horse) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 50kDa |
細(xì)胞定位 | 細(xì)胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human MRS2: 201-300/443 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
MRS2 is a magnesium transporter that may mediate the influx of magnesium into the mitochondrial matrix. There are 3 isoforms produced by alternative splicing. Function: Magnesium transporter that may mediate the influx of magnesium into the mitochondrial matrix. Subcellular Location: Mitochondrion inner membrane; Multi-pass membrane protein (by similarity). Similarity: Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family. SWISS: Q9HD23 Gene ID: 57380 Database links: Entrez Gene: 57380 Human Entrez Gene: 380836 Mouse SwissProt: Q9HD23 Human SwissProt: Q5NCE8 Mouse Unigene: 719256 Human |
產(chǎn)品圖片 |
Sample: Pancreas (mouse) Lysate at 40 ug
Primary: Anti- MRS2(bs-17836R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 50 kD
Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Mouse Heart tissue lysates
Lane 3: Rat Muscle tissue lysates
Lane 4: Rat Heart tissue lysates
Primary: Anti-MRS2 (bs-17836R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MRS2 ) Polyclonal Antibody, Unconjugated (bs-17836R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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