| 產(chǎn)品編號 | bs-17702R |
| 英文名稱 | Rabbit Anti-MOCS3 antibody |
| 中文名稱 | 鉬輔因子合成蛋白3抗體 |
| 別 名 | Adenylyltransferase MOCS3; dJ914P20.3; MGC9252; MOCS 3; MOCS3; MOCS3_HUMAN; Molybdenum cofactor synthesis 3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; Molybdopterin-synthase sulfurtransferase; MPT synthase sulfurylase; Sulfur carrier protein MOCS2A adenylyltransferase; Sulfur carrier protein MOCS2A sulfurtransferase; Sulfurtransferase MOCS3; UBA 4; UBA4 ubiquitin activating enzyme E1 homolog; UBA4. |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MOCS3: 11-110/460 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Molybdenum cofactor (MoCo) is necessary for the function of all molybdoenzymes. The protein encoded by this gene adenylates and activates molybdopterin synthase, an enzyme required for biosynthesis of MoCo. This gene contains no introns. A pseudogene of this gene is present on chromosome 14. [provided by RefSeq, Nov 2012] Function: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. Subunit: Interacts with NFS1. Subcellular Location: Cytoplasm > cytosol. Similarity: In the N-terminal section; belongs to the hesA/moeB/thiF family. UBA4 subfamily. Contains 1 rhodanese domain. SWISS: O95396 Gene ID: 27304 Database links: Entrez Gene: 27304 Human Entrez Gene: 69372 Mouse GenBank: BC015939 Human Omim: 609277 Human SwissProt: O95396 Human SwissProt: A2BDX3 Mouse Unigene: 159410 Human Unigene: 482182 Mouse |
| 產(chǎn)品圖片 |
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-MOCS3 (bs-17702R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
