| 產(chǎn)品編號 | bs-17389R |
| 英文名稱 | Rabbit Anti-HS2ST1 antibody |
| 中文名稱 | HS2ST1蛋白抗體 |
| 別 名 | 2-O-sulfotransferase; 2OST; dJ604K5.2; FLJ11317; heparan sulfate 2 O sulfotransferase 1; Heparan sulfate 2-O-sulfotransferase 1; HS2ST_HUMAN; hs2st1; KIAA0448; MGC131986. |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)運蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Zebrafish,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HS2ST1: 221-320/356 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Heparan sulfate structures, which are responsible for executing multiple biologic activities, are generated and regulated by heparan sulfate biosynthetic enzymes. HS2ST1 is a 356 amino acid single-pass type II membrane protein that localizes to the golgi apparatus and belongs to the sulfotransferase 3 family. Expressed as multiple alternatively spliced isoforms, HS2ST1 functions to catalyze the transfer of sulfate groups to hexuronic acid residues within maturing heparan sulfate (HS), an event which is crucial for proper HS-related ligand binding and signaling processes. HS2ST1 is subject to post-translational N-glycosylation and, in addition to its role in HS function, may be involved in proper kidney formation. Function: Catalyzes the transfer of sulfate to the C2-position of selected hexuronic acid residues within the maturing heparan sulfate (HS). 2-O-sulfation within HS, particularly of iduronate residues, is essential for HS to participate in a variety of high-affinity ligand-binding interactions and signaling processes. Mediates 2-O-sulfation of both L-iduronyl and D-glucuronyl residues. Subcellular Location: Golgi apparatus membrane. Post-translational modifications: N-glycosylated. Similarity: Belongs to the sulfotransferase 3 family. SWISS: Q7LGA3 Gene ID: 9653 Database links: Entrez Gene: 9653 Human Entrez Gene: 23908 Mouse GenBank: AA987571.1 Human SwissProt: Q7LGA3 Human SwissProt: Q8R3H7 Mouse Unigene: 137733 Human Unigene: 726055 Human Unigene: 12863 Mouse Unigene: 465667 Mouse |
| 產(chǎn)品圖片 |
Sample:
HepG2(Human) Cell Lysate at 30 ug
Lung (Mouse) Lysate at 40 ug
Primary: Anti-HS2ST1 (bs-17389R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 52 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
