| 產(chǎn)品編號(hào) | bs-17285R |
| 英文名稱(chēng) | Rabbit Anti-Schlafen 8 antibody |
| 中文名稱(chēng) | Schlafen 8蛋白抗體 |
| 別 名 | Slfn8; SLFN8_MOUSE; Schlafen family member 8; mSLFN8; Slfn8 pending. |
| 研究領(lǐng)域 | 細(xì)胞生物 t-淋巴細(xì)胞 表觀遺傳學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1ug/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 104kDa |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Schlafen 8: 831-910/910 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The regulatory networks governing development and differentiation of hematopoietic cells are incompletely understood. Members of the Schlafen protein family have been implicated in the regulation of cell growth and T cell development. Four new members, Schlafen 5, Schlafen 8, Schlafen 9 and Schlafen 10, which belong to a distinct subgroup within this gene family have been identified and chromosomally mapped. The characteristic feature of these proteins is the presence of sequence motifs identifying them as distinct members of the superfamily I of DNA/RNA helicases. SWISS: B1ARD8 Gene ID: 276950 Database links: Entrez Gene: 276950 Mouse SwissProt: B1ARD8 Mouse |
| 產(chǎn)品圖片 |
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-Schlafen 8 antibody (bs-17285R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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