| 產(chǎn)品編號(hào) | bs-17109R |
| 英文名稱 | Rabbit Anti-LAT2 antibody |
| 中文名稱 | L型氨基酸轉(zhuǎn)運(yùn)蛋白2抗體 |
| 別 名 | hLAT2; integral membrane protein E16H; L type amino acid transporter 2; L-type amino acid transporter 2; Large neutral amino acids transporter small subunit 2; LAT2_HUMAN; LPI PC1; SLC7A8; solute carrier family 7 (amino acid transporter light chain, L system), member 8; solute carrier family 7 (amino acid transporter, L type), member 8; solute carrier family 7 (cationic amino acid transporter, y+ system), member 8; Solute carrier family 7 member 8. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Pig,Sheep,Dog,Horse,Orangutan) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 61kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LAT2: 1-100/353 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
L-amino acid transporter protein-2 (LAT2), a non-glycosylated membrane protein, complexes with CD98 to contribute to readsorption of neutral amino acids in renal epithelia and blood-tissue barriers. The gene encoding LAT2 is expressed primarily in the kidney, but also to a lesser extent in placenta, brain, liver, spleen, skeletal muscle, heart, small intestine, and lung. Transfection with the antisense sequence of LAT2 suggests that LAT2 expression plays a major role in net basolateral efflux of cysteine, and points to LAT2 as a candidate gene to modulate cysteine reabsorption. In addition, the CD98/LAT2 heterodimer associates with Integrin ∫1 in intestinal epithelial cells, where ligand binding to CD98 and another cell surface molecule, ICAM-1 differentially regulates LAT2 activity, suggesting a novel mechanism by which events like cell adhesion may affect amino acid transport activity. Function: Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney. Subcellular Location: Cytoplasm. Basolateral cell membrane. Localized to the cytoplasm when expressed alone but when coexpressed with SLC3A2/4F2hc, is localized to the plasma membrane. Colocalized with SLC3A2/4F2hc at the basolateral membrane of kidney cortex proximal tubules and small intestine epithelia of the villi. Tissue Specificity: Strongest expression is observed in kidney and moderate expression in placenta and brain, followed by liver, prostate, testis, ovary, lymph node, thymus, spleen, skeletal muscle and heart. Also expressed in fetal liver as well as in the retinal pigment epithelial cell line ARPE-19 and the intestinal epithelial cell line Caco-2. Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. SWISS: Q9UHI5 Gene ID: 23428 Database links: Entrez Gene: 23428 Human Entrez Gene: 50934 Mouse Entrez Gene: 100009408 Rabbit Omim: 604235 Human SwissProt: Q9UHI5 Human SwissProt: Q9QXW9 Mouse SwissProt: Q5RAE3 Orangutan SwissProt: Q9N1Q4 Rabbit Unigene: 596643 Human Unigene: 276831 Mouse Unigene: 2421 Rabbit Unigene: 82734 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LAT2) Polyclonal Antibody, Unconjugated (bs-17109R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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