| 產(chǎn)品編號 | bs-16712R |
| 英文名稱 | Rabbit Anti-Trehalase antibody |
| 中文名稱 | 海藻糖酶抗體 |
| 別 名 | Alpha alpha trehalase; Alpha alpha trehalose glucohydrolase; Alpha; alpha-trehalase; alpha-trehalose glucohydrolase; Brush border membrane glycoprotein; MGC129621; TRE; TREA; TREA_HUMAN; TREH; Trehalase. |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 64kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Trehalase: 81-180/583 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Trehalase, also known as TREH, TREA or α,α-trehalose glucohydrolase, is a 583 amino acid protein belonging to the glycosyl hydrolase 37 family. Localizing to cell membrane and lipid-anchor, Trehalase is expressed in kidney, liver, and small intestine. Trehalase hydrolyses ingested trehalose, a disaccharide formed by two glucose molecules found mainly in insects, fungi, and plants, into cellular substrate glucose. Isolated trehalose intolerance due to deficiencies of Trehalase can result in gastrointestinal symptoms. Trehalase may also be a marker for renal tubular damage, and may contain an N-terminal signal peptide, five potential N-glycosylation sites, and a C-terminal hydrophobic region for glycosylphosphatidylinositol (GPI) attachment. Existing as two alternatively spliced isoforms, the gene encoding Trehalase maps to human chromosome 11q23.3. Function: Intestinal trehalase is probably involved in the hydrolysis of ingested trehalose. Subcellular Location: Cell membrane. DISEASE: Note=Deficiency of TREH results in isolated trehalose intolerance that causes gastrointestinal symptoms after ingestion of edible mushrooms. Similarity: Sequence similaritiesBelongs to the glycosyl hydrolase 37 family. SWISS: O43280 Gene ID: 11181 Database links: Entrez Gene: 11181 Human Omim: 275360 Human SwissProt: O43280 Human Unigene: 129712 Human |
| 產(chǎn)品圖片 |
Tissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Trehalase Polyclonal Antibody, Unconjugated(bs-16712R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
