| 產(chǎn)品編號 | bs-16619R |
| 英文名稱 | Rabbit Anti-IME1 antibody |
| 中文名稱 | 減數(shù)分裂誘導(dǎo)蛋白IME1抗體 |
| 別 名 | Meiosis inducing protein; IME1_YEAST; YJR094C; Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). 釀酒酵母; |
| 研究領(lǐng)域 | 細胞生物 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Saccharomyces cerevisiae) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Saccharomyces cerevisiae IME1: 301-360/360 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Meiosis-inducing protein (IME1) is a master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p. IME1 is required for sporulation and for early sporulation-specific genes expression. It is a positive regulator of SME1/IME2 expression. Function: Transcription factor required for sporulation and for early sporulation-specific genes expression. Positive regulator of SME1/IME2 expression. Directly activates expression of SLZ1 during meiosis. Subunit: Interacts with UME6. Subcellular Location: Nuclear SWISS: P21190 Gene ID: N/A Database links: Entrez Gene: 853556 Saccharomyces cerevisiae SwissProt: P21190 Saccharomyces cerevisiae |
| 產(chǎn)品圖片 |
Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IME1 Polyclonal Antibody, Unconjugated(bs-16619R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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