產(chǎn)品編號 | bs-16590R |
英文名稱 | Rabbit Anti-TRAPPCL2 antibody |
中文名稱 | HSPC176蛋白抗體 |
別 名 | FLJ44827; Hematopoietic stem/progenitor cells 176; HSPC176; MGC111156; TPC2L_HUMAN; Trafficking protein particle complex 2 like; Trafficking protein particle complex subunit 2 like protein. |
研究領域 | 細胞生物 信號轉(zhuǎn)導 轉(zhuǎn)運蛋白 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Mouse (predicted: Human,Rat,Pig,Cow,Dog,Horse) |
產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 16kDa |
細胞定位 | 細胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human TRAPPCL2: 21-80/140 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
TRAPPC2L may play a role in vesicular transport from endoplasmic reticulum to Golgi. Function: May play a role in vesicular transport from endoplasmic reticulum to Golgi. Subunit: Component of the multisubunit TRAPP (transport protein particle) complex, which includes at least TRAPPC2, TRAPPC2L, TRAPPC3, TRAPPC3L, TRAPPC4, TRAPPC5, TRAPPC8, TRAPPC9, TRAPPC10, TRAPPC11 and TRAPPC12. Interacts with the heterodimer TRAPPC3-TRAPPC6A. Interacts with TRAPPC6A. Subcellular Location: Cytoplasmic, Endoplasmic reticulum and Golgi Apparatus Tissue Specificity: Expressed in testis, liver, bladder, lung, spleen and brain, several cell lines and primary chondrocytes cell line. Similarity: Belongs to the TRAPP small subunits family. Sedlin subfamily. SWISS: Q9UL33 Gene ID: 51693 Database links: Entrez Gene: 51693 Human Entrez Gene: 59005 Mouse Omim: 610970 Human SwissProt: Q9UL33 Human SwissProt: Q9JME7 Mouse |
產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRAPPCL2) Polyclonal Antibody, Unconjugated (bs-16590R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |