| 產品編號 | bs-16565R |
| 英文名稱 | Rabbit Anti-TRAM1 antibody |
| 中文名稱 | 相關易位膜蛋白1抗體 |
| 別 名 | PNAS 8; PNAS8; PRO1292; TRAM 1; TRAM; TRAM1_HUMAN; TRAMP; Translocating chain associated membrane protein 1; Translocating chain associating membrane protein; Translocation associated membrane protein 1; Translocation associating membrane protein 1. |
| 研究領域 | 細胞生物 信號轉導 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 43kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TRAM1: 61-160/374 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
TRAM1 belongs to the TRAM family. It stimulates or is required for the translocation of secretory proteins across the endoplasmic reticulum membrane. Function: Stimulatory or required for the translocation of secretory proteins across the ER membrane. Subunit: Interacts with human cytomegalovirus/HHV-5 protein US2. Subcellular Location: Endoplasmic reticulum membrane; Multipass membrane protein Similarity: Belongs to the TRAM family. Contains 1 TLC (TRAM/LAG1/CLN8) domain. SWISS: Q15629 Gene ID: 23471 Database links: Entrez Gene: 23471 Human Omim: 605190 Human SwissProt: Q15629 Human |
| 產品圖片 |
Tissue/cell: Human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TRAM1 Polyclonal Antibody, Unconjugated(bs-16565R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
