產(chǎn)品編號 | bs-16479R |
英文名稱 | Rabbit Anti-ZNF774 antibody |
中文名稱 | 鋅指蛋白774抗體 |
別 名 | Zinc finger protein 774; ZN774_HUMAN; ZNF774. |
研究領域 | 轉錄調(diào)節(jié)因子 鋅指蛋白 表觀遺傳學 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human |
產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 55kDa |
細胞定位 | 細胞核 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ZNF774: 31-130/483 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
ZNF774 (zinc finger protein 774) is a 483 amino acid protein that localizes to the nucleus and contains 12 C2H2-type zinc fingers and a KRAB domain. One of several members of the Krüppel C2H2-type zinc-finger protein family, ZNF774 is thought to be involved in transcriptional regulation events. The gene encoding ZNF774 maps to human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome. Angelman syndrome, Prader-Willi syndrome, Tay-Sachs disease and Marfan syndrome are all associated with defects in chromosome 15-localized genes. Function: May be involved in transcriptional regulation. Subcellular Location: Nucleus. Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 12 C2H2-type zinc fingers. Contains 1 KRAB domain. SWISS: Q6NX45 Gene ID: 342132 Database links: Entrez Gene: 342132 Human SwissProt: Q6NX45 Human Unigene: 55307 Human |
產(chǎn)品圖片 |
Sample:Lovo Cell Lysate at 40 ug
Primary: Anti-ZNF774 (bs-16479R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |