| 產(chǎn)品編號 | bs-16311R |
| 英文名稱 | Rabbit Anti-GRF2 antibody |
| 中文名稱 | G蛋白偶聯(lián)受體釋放因子2抗體 |
| 別 名 | C3G; C3G protein; CRK SH3 binding GNRP; CRK SH3-binding GNRP; DKFZp781P1719; GRF 2; GRF2; Guanine nucleotide releasing factor 2 (specific for crk proto oncogene); Guanine nucleotide releasing factor 2; Guanine nucleotide-releasing factor 2; OTTHUMP00000064558; Protein C3G; Rap guanine nucleotide exchange factor (GEF) 1; Rap guanine nucleotide exchange factor 1; RAPGEF 1; RAPGEF1; RPGF1_HUMAN. |
| 研究領域 | 細胞生物 信號轉(zhuǎn)導 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Sheep,Cow) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 140kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GRF2: 20-120/1237 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] Function: Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Tissue Specificity: Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver. Similarity: Contains 1 N-terminal Ras-GEF domain. Contains 1 Ras-GEF domain. SWISS: O14827 Gene ID: 2889 Database links: Entrez Gene: 2889 Human Entrez Gene: 107746 Mouse Omim: 600303 Human SwissProt: Q13905 Human Unigene: 127897 Human Unigene: 298274 Mouse |
| 產(chǎn)品圖片 |
Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-GRF2 (bs-16311R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140 kD
Observed band size: 120 kD
Sample:
Lane 1: Human MCF-7 cell lysates
Lane 2: Human HL-60 cell lysates
Lane 3: Human JAR cell lysates
Lane 4: Human HeLa cell lysates
Primary: Anti-GRF2 (bs-16311R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140 kDa
Observed band size: 140 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
