產(chǎn)品編號 | bs-16109R |
英文名稱 | Rabbit Anti-FLJ20259 antibody |
中文名稱 | 富含谷氨酰胺1蛋白/Glutamine-rich 1抗體 |
別 名 | FLJ20259 protein; Glutamine-rich protein 1; QRIC1_HUMAN; QRICH1. |
研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 細(xì)胞凋亡 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 87kDa |
細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human FLJ20259: 601-700/776 |
亞 型 | |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Glutamine-rich 1 is a 776 amino acid protein containing one CARD domain, which is normally found in proteins that are related to inflammation and apoptosis processes. CARD domains are found in a wide variety of proteins including kinases, caspases, and helicases. The gene encoding Glutamine-rich 1 maps to human chromosome 3. Chromosome 3 is made up of about 214 million bases encoding over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. Key tumor suppressing genes on chromosome 3 include those that encode the apoptosis mediator RASSF1, the cell migration regulator HYAL1 and the angiogenesis suppressor SEMA3B. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth Disease are a few of the numerous genetic diseases associated with chromosome 3. Similarity: Contains 1 CARD domain. SWISS: Q2TAL8 Gene ID: 54870 Database links: Entrez Gene: 54870 Human SwissProt: Q2TAL8 Human Unigene: 297389 Human |
產(chǎn)品圖片 |
Sample:
HL60(Human) Cell Lysate at 30 ug
Primary: Anti-FLJ20259 (bs-16109R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 87 kD
Observed band size: 87 kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FLJ20259) Polyclonal Antibody, Unconjugated (bs-16109R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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