| 產(chǎn)品編號 | bs-16081R |
| 英文名稱 | Rabbit Anti-FGD5 antibody |
| 中文名稱 | FGD5蛋白抗體 |
| 別 名 | FYVE, RhoGEF and PH domain containing protein 5; ZFYVE23; FGD5_HUMAN; Zinc finger FYVE domain containing protein 23. |
| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human,Mouse,Rat,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 160kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FGD5: 1301-1462/1462 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
FGD5 is a 1,462 amino acid cytosolic protein that contains a DH domain, a FYVE-type zinc finger and two PH domains. FGD family members encode guanine nucleotide exchange factors that specifically activate the Rho GTPase Cdc42. All FGD proteins contain equivalent signaling domains and a conserved structural organization, which strongly suggests that these signaling domains form a canonical core structure for members of the FGD family of RhoGEF proteins. These proteins also control essential signals required during embryonic development. Function: FGD5 may activate CDC42, a member of the Ras like family of Rho and Rac proteins, by exchanging bound GDP for free GTP. It may also play a role in regulating the actin cytoskeleton and cell shape. FGD5 contains one DH (DBL-homology) domain one FYVE type zinc finger and two PH domains. There are two isoforms. Subcellular Location: Cytoplasm, cytoskeleton. Cell projection, ruffle membrane. Endoplasmic reticulum. Golgi apparatus. Early endosome. Note=In peripheral membrane ruffles, colocolizes with F-actin. In confluent HUVECs, detected at cell-cell-contact sites where it colocalizes with vascular endothelial cadherin/CDH5. Tissue Specificity: Expressed in endothelial cells (at protein level). Similarity: Contains 1 DH (DBL-homology) domain. Contains 1 FYVE-type zinc finger. Contains 2 PH domains. SWISS: Q6ZNL6 Gene ID: 152273 Database links: Entrez Gene: 152273 Human Entrez Gene: 232237 Mouse Omim: 614788 Human SwissProt: Q6ZNL6 Human SwissProt: Q80UZ0 Mouse Unigene: 412406 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGD5) Polyclonal Antibody, Unconjugated (bs-16081R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
