| 產(chǎn)品編號 | bs-14677R |
| 英文名稱 | Rabbit Anti-FIAA antibody |
| 中文名稱 | FIAA蛋白抗體 |
| 別 名 | F1A alpha; FEM1B_HUMAN; Fem 1 like in apoptotic pathway protein alpha; FEM-1 homolog b; FEM-1-like death receptor binding protein; FEM1 beta; FIAA; Protein FEM 1 homolog b. |
| 研究領(lǐng)域 | 細胞生物 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 70kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FIAA: 1-100/627 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes an ankyrin repeat protein that belongs to the death receptor-associated family of proteins and plays a role in mediating apoptosis. The encoded protein is also thought to function in the replication stress-induced checkpoint signaling pathway via interaction with checkpoint kinase 1. [provided by RefSeq, Aug 2013] Subcellular Location: Cytoplasm. Nucleus. Note: In the nucleus, the protein level increased slightly after camptothecin (CPT) treatment. Associated with chromatin. Tissue Specificity: Widely expressed. Highly expressed in testis. Weakly expressed in other tissues. Similarity: Belongs to the fem-1 family. Contains 8 ANK repeats. Contains 1 TPR repeat. SWISS: Q9UK73 Gene ID: 10116 Database links: Entrez Gene: 10116 Human Entrez Gene: 415559 Chicken Entrez Gene: 14155 Mouse Omim: 613539 Human SwissProt: Q5ZM55 Chicken SwissProt: Q9UK73 Human SwissProt: Q9Z2G0 Mouse Unigene: 362733 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FIAA) Polyclonal Antibody, Unconjugated (bs-14677R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
