| 產(chǎn)品編號(hào) | bs-14596R |
| 英文名稱 | Rabbit Anti-ENO4 antibody |
| 中文名稱 | ENO4蛋白抗體 |
| 別 名 | C10orf134; AC023283.3; C10orf134; eno4; ENO4_HUMAN; Enolase 4; Enolase family member 4; Enolase-like protein ENO4. |
| 研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Rabbit,Sheep,Cow) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 69kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ENO4: 401-500/628 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Enolases have been characterized as highly conserved cytoplasmic glycolytic enzymes that may be involved in differentiation. Three isoenzymes have been identified, alpha enolase, beta enolase and gamma enolase. alpha enolase expression has been detected on most tissues, whereas betaenolase is expressed predominantly in muscle tissue and gamma enolase is detected only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. ENO4 (Enolase-like protein ENO4), also known as C10orf134, is a 628 amino acid protein that belongs to the Enolase family. ENO4 is expressed as two isoforms produced by alternative splicing and is encoded by a gene mapping to human chromosome 10q25.3 and murine chromosome 19 D3. Similarity: Belongs to the enolase family. SWISS: A6NNW6 Gene ID: 387712 Database links: Entrez Gene: 387712 Human Entrez Gene: 226265 Mouse Omim: 131375 Human SwissProt: A6NNW6 Human SwissProt: Q8C042 Mouse Unigene: 696001 Human Unigene: 103154 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ENO4) Polyclonal Antibody, Unconjugated (bs-14596R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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