| 產(chǎn)品編號 | bs-10343R |
| 英文名稱 | Rabbit Anti-Phosphor-PPP3CA (Ser469) antibody |
| 中文名稱 | 磷酸化鈣調(diào)磷酸酶A/鈣依賴磷酸酶A抗體 |
| 別 名 | PPP3CA(Phosphor S469); PPP3CA(Phosphor Ser469); Calcineurin A alpha; CalcineurinA; Calcineurin-A; Calcineurin A; Calmodulin dependent calcineurin A subunit alpha isoform; CALN; CALNA 1; CALNA; CALNA1; CAM PRP catalytic subunit; CCN 1; CCN1; CNA 1; CNA; CNA1; PPP2B; Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform; Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform; PP2BA_HUMAN; CAM-PRP catalytic subunit; Calmodulin-dependent calcineurin A subunit alpha isoform; protein phosphatase 2B, catalytic subunit, alpha isoform; serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Cow,Chicken,GuineaPig) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:25,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 57kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human PPP3CA around the phosphorylation site of Ser469: 421-521/521 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Calcineurin, a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. This metalloenzyme, also known as phosphatase 2B, is a heterodimer composed of a calmodulin binding, catalytic alpha subunit 61 kDa, calcineurin A) and calcium binding beta subunit (18 kDa, calcineurin B). The Ca2+ binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. The presence of 2 different calcineurin B isoforms (beta 1 and beta 2) has been reported in rat testis. The catalytic subunit of calcineurin, calcineurin A, isolated from different tissues or different organisms, exhibits some immunological heterogeneity. Further, there are at least 2 isoforms of calcineurin A in bovine brain (alpha 1 and alpha 2, 61 and 59 kDa, respectively). Function: Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1. Subcellular Location: Nucleus. Note=Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle. Tissue Specificity: Interacts with CHP1 and CHP2. Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity. Interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3. Interacts with DNM1L; the interaction dephosphorylates DNM1L and regulates its translocation to mitochondria. Interacts with CMYA5; this interaction represses calcineurin activity in muscle. Similarity: Belongs to the PPP phosphatase family. PP-2B subfamily. SWISS: Q08209 Gene ID: 5530 Database links: Entrez Gene: 5530 Human Entrez Gene: 19055 Mouse Omim: 114105 Human SwissProt: Q08209 Human SwissProt: P63328 Mouse Unigene: 435512 Human Unigene: 331389 Mouse Unigene: 6866 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP3CA (Ser469)) Polyclonal Antibody, Unconjugated (bs-10343R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phosphor-PPP3CA (Ser469)) polyclonal Antibody, Unconjugated (bs-10343R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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