產品編號 | bs-13713R |
英文名稱 | Rabbit Anti-AJAP1 antibody |
中文名稱 | 粘合連接相關蛋白1抗體 |
別 名 | Adherens junction-associated protein 1; Adherens junction-associated protein 1; Ajap1; AJAP1_HUMAN; Membrane protein shrew-1; MOT8; SHREW-1; SHREW1 |
研究領域 | 腫瘤 細胞生物 信號轉導 細胞粘附分子 細胞膜蛋白 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human,Mouse,Rat (predicted: Dog,Horse) |
產品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 46kDa |
細胞定位 | 細胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human AJAP1: 341-411/411 |
亞 型 | |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
Plays a role in cell adhesion and cell migration. Subcellular Location: Basolateral cell membrane. Apical cell membrane. Cell junction > adherens junction. Mainly basolateral. Localization is mediated by AP1M2. Tissue Specificity: Expressed in uterus and pancreas (at protein level). Post-translational modifications: Thr-237 and Ser-239 may be phosphorylated; however as this position is probably extracellular, the in vivo relevance is not proven. SWISS: Q9UKB5 Gene ID: 55966 Database links: Entrez Gene: 55966 Human SwissProt: Q9UKB5 Human Unigene: 25924 Human |
產品圖片 |
Sample:
Lane 1: U87MG (Human) Cell Lysate at 30 ug
Lane 2: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 3: Cerebrum (Mouse) Lysate at 40 ug
Lane 4: Cerebrum (Rat) Lysate at 40 ug
Lane 5: Uterus (Mouse) Lysate at 40 ug
Lane 6: Uterus (Rat) Lysate at 40 ug
Lane 7: Pancreas (Mouse) Lysate at 40 ug
Lane 8: Pancreas (Rat) Lysate at 40 ug
Lane 9: Kidney (Mouse) Lysate at 40 ug
Primary: Anti-AJAP1 (bs-13713R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |