| 產(chǎn)品編號 | bs-13610R |
| 英文名稱 | Rabbit Anti-HCG3 antibody |
| 中文名稱 | HCG3蛋白抗體 |
| 別 名 | DnaJ (Hsp40) homolog subfamily B member 3; DnaJ homolog subfamily B member 3; Dnajb3; DNJB3_HUMAN; HCG3 gene; HCG3 protein; Hypothetical protein LOC414061; MGC26879; Putative uncharacterized protein tmp_locus_21; Tmp_locus_21. |
| 研究領(lǐng)域 | 細胞生物 發(fā)育生物學 信號轉(zhuǎn)導 干細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 17kDa |
| 細胞定位 | 細胞外基質(zhì) |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HCG3: 1-100/145 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The DnaJ family is one of the largest of all the chaperone families and has evolved with diverse cellular localization and functions. Members of the DnaJ family are characterized by the presence of the J domain. DnaJ heat shock induced proteins are from the bacterium Escherichia coli and are under the control of the htpR regulatory protein. The DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis. Proteins of the DnaJ family contain cysteine rich regions that are composed of zinc fingers that form a peptide binding domain responsible for chaperone function. DnaJ proteins are important mediators of proteolysis and are involved in the regulation of protein degradation, exocytosis and endocytosis. Function: May operate as a co-chaperone of the male germ cell- and haploid stage-specific Hsp70 proteins. Tissue Specificity: Expressed in sperm (at protein level). Similarity: Contains 1 J domain. SWISS: Q8WWF6 Gene ID: 414061 Database links: Entrez Gene: 414061 Human SwissProt: Q8WWF6 Human Unigene: 725533 Human |
| 產(chǎn)品圖片 |
Sample: testis (Mouse) Lysate at 40 ug
Primary: Anti-HCG3(bs-13610R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 19 kD
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
