| 產品編號 | bs-15391R |
| 英文名稱 | Rabbit Anti-GPR89A antibody |
| 中文名稱 | G蛋白偶聯(lián)受體GPR89A蛋白抗體 |
| 別 名 | CGI-13; Golgi pH regulator A; Golgi pH regulator B; GPHR; GPHRA; GPHRB; GPR89; GPR89A; GPR89B; HSPC201; Protein GPR89A; Protein GPR89B; Putative MAPK-activating protein PM01; Putative NF-kappa-B-activating protein 90; SH120; UNQ192/PRO218; GPHRA_HUMAN. |
| 研究領域 | 細胞生物 免疫學 信號轉導 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Sheep,Cow,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 53kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR89A: 221-320/445 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
GPR89A is a nearly identical copy of the GPR89B gene (MIM612806). Function: Voltage dependent anion channel required for acidification and functions of the Golgi apparatus that may function in counter-ion conductance. Subunit: Homotrimer. Subcellular Location: Golgi apparatus membrane; Multi-pass membrane protein. Tissue Specificity: Ubiquitous. Similarity: Belongs to the Golgi pH regulator (TC 1.A.38) family. SWISS: B7ZAQ6 Gene ID: 653519 Database links: Entrez Gene: 653519 Human Entrez Gene: 67549 Mouse Omim: 612821 Human SwissProt: B7ZAQ6 Human SwissProt: Q8BS95 Mouse |
| 產品圖片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Primary: Anti-GPR89A (bs-15391R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 50 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR89A) Polyclonal Antibody, Unconjugated (bs-15391R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
