| 產(chǎn)品編號 | bs-13380R |
| 英文名稱 | Rabbit Anti-GLT28D1 antibody |
| 中文名稱 | 糖基轉(zhuǎn)移酶28家族1抗體 |
| 別 名 | ALG13; GLT28D1; ALG13_HUMAN; Asparagine linked glycosylation 13 homolog (S. cerevisiae); Asparagine-linked glycosylation 13 homolog; CXorf45; FLJ23018; FLJ31785; GLT28D1; Glycosyltransferase 28 domain containing protein 1; Glycosyltransferase 28 domain-containing protein 1; Hematopoietic stem/progenitor cells protein MDS031; MDS031; MGC12423; UDP N acetylglucosamine transferase subunit ALG13 homolog; UDP-N-acetylglucosamine transferase subunit ALG13 homolog; YGL047W. |
| 研究領域 | 細胞生物 信號轉(zhuǎn)導 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 126kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GLT28D1/ALG13: 21-120/1137 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ALG13 is a 1,137 amino acid protein belonging to the glycosyltransferase 28 family. Encoded by a gene that maps to human chromosome Xq23, ALG13 is a subunit of a bipartite UDP-N-acetylglucosamine transferase and plays a role in protein folding regulation and stabilization. ALG13 contains one OTU domain, one TudorSN domain, and exists as four alternatively spliced isoforms. Heterodimerizing with ALG14, ALG13 forms a UDP-GlcNAc glycosyltransferase, which catalyzes the second sugar addition of the oligosaccharide precursor in endoplasmic reticulum (ER) N-linked glycosylation. ALG13 localizes to ER and may be recruited to the cytosolic face of the membrane by interacting with ALG14. Function: Isoform 2 may be involved in protein N-glycosylation, second step of the dolichol-linked oligosaccharide pathway. Subunit: Isoform 2 may interact with ALG14. Subcellular Location: Endoplasmic reticulum. Could be recruited to the cytosolic face of the endoplasmic reticulum membrane through its interaction with ALG14. Similarity: Belongs to the glycosyltransferase 28 family. Contains 1 OTU domain. Contains 1 Tudor domain. SWISS: Q9NP73 Gene ID: 79868 Database links: Entrez Gene: 79868 Human Omim: 300776 Human SwissProt: Q9NP73 Human Unigene: 443061 Human |
| 產(chǎn)品圖片 |
Tissue/cell: Rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GLT28D1 Polyclonal Antibody, Unconjugated(bs-13380R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
