| 產(chǎn)品編號 | bs-13372R |
| 英文名稱 | Rabbit Anti-GLEPP1 antibody |
| 中文名稱 | 腎小球上皮蛋白1抗體 |
| 別 名 | FMI; GLEPP 1; Glomerular epithelial protein 1; hPTP-J; Pancreatic carcinoma phosphatase 2; PCP-2; PCP2; Phosphotyrosine phosphatase U2; Protein tyrosine phosphatase PTP U2; Protein tyrosine phosphatase PTPU2; Protein tyrosine phosphatase receptor type O; Protein tyrosine phosphatase U2; Protein-tyrosine phosphatase J; Protein-tyrosine phosphatase pi; Protein-tyrosine phosphatase receptor omicron; PTP pi; PTP U2; PTP-J; PTP-RO; PTP-U2; PTPase U2; PTPRO; PTPRO_HUMAN; PTPROt; PTPU 2; PTPU2; R-PTP-O; R-PTP-psi; Receptor type protein tyrosine phosphatase O; Receptor type tyrosine protein phosphatase O; Receptor-type protein-tyrosine phosphatase psi; Receptor-type tyrosine-protein phosphatase O; Receptor-type tyrosine-protein phosphatase U. |
| 研究領(lǐng)域 | 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 135kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GLEPP1: 351-450/1216 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Protein phosphatases play critical roles in the regulation of signal transduction pathways. The family can be separated into three general categories, which are distinguished on the basis of substrate specificity. The first and largest category, termed protein tyrosine phosphatases or PTPs, includes transmembrane proteins, membrane associated proteins and proteins that localize to nuclei. The second category of protein phosphatases dephosphorylate proteins on phosphoserine and phosphothreonine residues, whereas the third category of protein phosphatases exhibit dual specificities and can dephosphorylate proteins on phosphotyrosine and phosphoserine/phosphothreonine residues. Glomerular epithelial protein 1 (GLEPP1), also designated protein tyrosine phosphatase U2 (PTPase U2) or receptor-type tyrosine-protein phosphatase O (PTPRO), belongs to the protein-tyrosine phosphatase family. GLEPP1 is a Type I membrane protein containing 8 fibronectin type-III domains and 1 tyrosine-protein phosphatase domain. GLEPP1 is expressed primarily in the glomeruli of the kidney, but is also detected in placenta, lung and brain. Function: Possesses tyrosine phosphatase activity. Subcellular Location: Membrane. Tissue Specificity: Glomerulus of kidney. Also detected in brain, lung and placenta. DISEASE: Defects in PTPRO are the cause of nephrotic syndrome type 6 (NPHS6) [MIM:614196]. NPHS6 is a renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. Similarity: Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily. Contains 8 fibronectin type-III domains. Contains 1 tyrosine-protein phosphatase domain. SWISS: Q16827 Gene ID: 5800 Database links: Entrez Gene: 5800 Human Omim: 600579 Human SwissProt: Q16827 Human Unigene: 160871 Human |
| 產(chǎn)品圖片 |
Tissue/cell: rat lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GGA2 Polyclonal Antibody, Unconjugated(bs-13344R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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