| 產(chǎn)品編號(hào) | bs-13283R |
| 英文名稱 | Rabbit Anti-GAPex5 antibody |
| 中文名稱 | Rab5激活蛋白6抗體 |
| 別 名 | GAPEX 5; GAPVD1; GTPase activating protein and VPS9 domains 1; KIAA1521; Rab5 activating protein 6; RAP6; GAPD1_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) G蛋白偶聯(lián)受體 新陳代謝 G蛋白信號(hào) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human,Mouse,Rat,Rabbit,Sheep,Cow,Chicken,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 145kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GAPex5/GAPVD1/RAP6: 1151-1250/1478 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
GAPVD1 is a 1478 amino acid peripheral membrane protein that acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF). GAPVD1 participates in many processes such as insulin receptor internalization, Glut4 trafficking and endocytosis. In addition, depletion of GAPVD1 leads to delayed EGFR degradation by mediating receptor ubiquination through its RGD domain, suggesting that it may be an important mediator of carcinogenesis resulting from Ras protein mutations. There are six isoforms of GAPVD1 that are produced as a result of alternative splicing events. Function: GAPex 5 plays a role in several processes such as endocytosis, insulin receptor internalisation and LC2A4/GLUT4 trafficking. It acts as both a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF). GAPex 5 is a GEF for the small G protein Rab31, promoting the exchange of GDP bound to Rab31 for GTP and thus regulating LC2A4/GLUT4 trafficking. In the absence of insulin, GAPex 5 maintains Rab31 in an active (GTP-bound) state, thereby promoting a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, keeping LC2A4/GLUT4 inside the cells. Following insulin stimulation, GAPex 5 is translocated to the plasma membrane, enabling the release of LC2A4/GLUT4 from the intracellular storage vesicles. GAPex 5 is also involved in EGFR trafficking and degradation, and also has GEF activity for Rab5 and GAP activity for Ras. Subunit: Interacts with TRIP10/CIP4 (By similarity). Interacts with RAB5A. Subcellular Location: Cell Membrane (peripheral membrane protein) and Cytoplasmic. Similarity: Belongs to the GAPVD1 family. Contains 1 Ras-GAP domain. Contains 1 VPS9 domain. SWISS: Q14C86 Gene ID: 26130 Database links: Entrez Gene: 26130 Human Omim: 611714 Human SwissProt: Q14C86 Human Unigene: 495134 Human |
