| 產(chǎn)品編號(hào) | bs-12998R |
| 英文名稱 | Rabbit Anti-DGAT2 antibody |
| 中文名稱 | 甘油二酯酰基轉(zhuǎn)移酶2抗體 |
| 別 名 | DGAT2_HUMAN; Diacylglycerol O acyltransferase like protein 2; Diacylglycerol O-acyltransferase 2; Diacylglycerol O-acyltransferase homolog 2 (mouse); Diacylglycerol O-acyltransferase homolog 2; Diacylglycerol O-acyltransferase-like protein 2 isoform 1; Diglyceride acyltransferase 2; DKFZp686A15125; GS1999full; HMFN1045. |
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Specific References (4) | bs-12998R has been referenced in 4 publications.
[IF=20.773] van Rijn JM et al. Intestinal Failure and Aberrant Lipid Metabolism in Patients With?DGAT1 Deficiency. Gastroenterology. 2018 Jul;155(1):130-143.e15. WB ; Human.
[IF=6.268] Jin, Yi. et al. Glutathione S-transferase Mu 2 inhibits hepatic steatosis via ASK1 suppression. COMMUN BIOL. Commun Biol. 2022 Apr;5(1):1-12 IF ; Human.
[IF=4.743] van Rijn JM et al. DGAT2 partially compensates for lipid-induced ER stress in human DGAT1-deficient intestinal stem cells. J Lipid Res. 2019 Jul 17. pii: jlr.M094201. WB ; Human.
[IF=2.559] Wang X et al. ER stress mediated degradation of diacylglycerol acyltransferase impairs mitochondrial functions in TMCO1 deficient cells. Biochem Biophys Res Commun. 2019 May 14;512(4):914-920. WB ; Human.
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| 研究領(lǐng)域 | 腫瘤 心血管 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-300
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 44kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DGAT2: 251-360/388 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Glucose and insulin are anabolic signals which upregulate the transcriptions of a series of lipogenic enzymes to convert excess carbohydrate into triglycerides for efficient energy storage. Acyl-coenzyme A:diacylglycerol acyltransferase, also known as DGAT1 and ARGP1, is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. DGAT1 catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT1 plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism, such as intestinal fat absorption, lipoprotein assembly, adipose tissue form-ation and lactation. DGAT2, which has no homology to DGAT1, differs from DGAT1 in that its activity has been shown to be inhibited by MgCl in an in vitro assay. DGAT2 is expressed primarily in liver and white adipose tissue, which suggests that it plays an important role in mammalian triglyceride metabolism. Function: Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. Subunit: Forms multimeric complexes consisting of several DGAT2 subunits (By similarity). Subcellular Location: Endoplasmic reticulum membrane. Tissue Specificity: Predominantly expressed in liver and white adipose tissue. Expressed at lower level in mammary gland, testis and peripheral blood leukocytes. Expressed in sebaceous glands of normal skin but decreased psoriatic skin. Similarity: Belongs to the diacylglycerol acyltransferase family. SWISS: Q96PD7 Gene ID: 84649 Database links: Entrez Gene: 84649 Human Entrez Gene: 67800 Mouse SwissProt: Q96PD7 Human SwissProt: Q9DCV3 Mouse |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Rat Liver tissue lysates
Primary: Anti-DGAT2 (bs-12998R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with DGAT2 Polyclonal Antibody, Unconjugated (bs-12998R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
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